Presenter: Sara Pérez Muñoz, Thermo Fisher Scientific, Spain Advancements in cell culture media development and transfection methods have led to improved cell culture and gene editing efficiency of most cell types. However, for sensitive cell types like PSCs, poor cell survival, recovery and efficient differentiation to neural cells as well as the later maintenance of neural cultures remain a significant challenge. Here we present the latest advancements to improve gene editing efficiency, cell survival and neural cell differentiation. The Neon™ NxT Electroporation System will be presented, highlighting its flexibility and ease of use while high transfection efficiency and cell viability are achieved. In the context of cell survival, we will introduce the versatile CultureCEPT™ Supplement and its excellent performance compared to existing recovery reagents. As the last step of the PSC culture and differentiation workflow, we will discuss the B-27 Plus Supplement and its advantages in maturation and long-term culture of neurons.
THERMO FISHER SCIENTIFIC INNOVATION SHOWCASE: THE LATEST ADVANCEMENT TO IMPROVE EFFICIENCY AND VIABILITY FROM GENE-EDITING PSCS TO NEURAL DIFFERENTIATION
